Composite

Part:BBa_K5066011:Design

Designed by: Abner Tseng   Group: iGEM24_GEMS-Taiwan   (2024-09-27)


Xpp81Aa-Cyt2Ba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1595
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1595
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1595
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1595
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1595
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Xpp81Aa1 enhances the performance of Cyt2Ba by providing another source of toxicity that works in a synergistic way between the two toxins.

Source

Engineered in GEMS Academy

References

Wu, J., Wei, L., He, J., Fu, K., Li, X., Jia, L., Wang, R., & Zhang, W. (2021). Characterization of a novel Bacillus thuringiensis toxin active against Aedes aegypti larvae. Acta tropica, 223, 106088. https://doi.org/10.1016/j.actatropica.2021.106088

Yu-Mei Xiong, Min-Hua Ling, Zheng-Dao Lan, Da-Cheng Wang, Cheng-Wu Chi,The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch,Toxicon, Volume 37, Issue 2, 1999, Pages 335-341, ISSN 0041-0101, https://doi.org/10.1016/S0041-0101(98)00176-7.

Deng, S. Q., Deng, M. Z., Chen, J. T., Zheng, L. L., & Peng, H. J. (2017). Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 37(6), 750–754. https://doi.org/10.3969/j.issn.1673-4254.2017.06.06

Bravo, A., Likitvivatanavong, S., Gill, S. S., & Soberón, M. (2011). Bacillus thuringiensis: A story of a successful bioinsecticide. Insect biochemistry and molecular biology, 41(7), 423–431. https://doi.org/10.1016/j.ibmb.2011.02.006